Coding

Part:BBa_K4835034:Design

Designed by: Kun Zhang   Group: iGEM23_XJU-China   (2023-10-11)


The protein-coding gene thrC can be used to translate threonine synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 109
    Illegal XhoI site found at 1277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 109
    Illegal NgoMIV site found at 1239
    Illegal AgeI site found at 252
    Illegal AgeI site found at 409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 217
    Illegal SapI.rc site found at 598
    Illegal SapI.rc site found at 937


Design Notes

If you want to use it in a project, you need to be careful not to affect other genes.


Source

Reference Genome: Enterobacter cloacae RS35


References